Callus induction, clonal propagation and in vitrogermplasm conservation of ‘hualtaco’ Loxopterygium huasangoSpruce ex Engl. (Anacardiaceae)

Loxopterygium huasango Spruce ex Engl., of the Anacardiaceae family, classified critically endangered, is a forest tree with considerable value in Seasonally Dry Tropical Forest (SDTF) northwest Peru and south Ecuador. The aim study was callus induction, propagation germplasm conservation using vitro tissue culture techniques. In seed germination (90%) obtained on Murashige Skoog (MS) medium supplemented 5.0 mg/L-1 GA3. cotyledonal nodes seedlings were used as explants to initiate growth apical shoots, IAA-GA3 interactions, without observing axillary buds. highest rate friable induction (100%) recorded MS containing 0.1 2,4-D cotyledons hypocotyls after 30 45 days culture. profuse roots formation only morphogenic response (10.8 13.8%) 0.5 IAA, IAA 2.0 BAP, KIN or 2iP 2iP. buds nodal segments achieved AgNO3. This method could be conserve elite genotypes, rapid production establishment cellular suspension for secondary compounds production.